Journal: Metabolic Engineering Communications

Article Title: Engineering Clostridium thermocellum for production of 2,3-butanediol from cellulose ☆

doi: 10.1016/j.mec.2025.e00269

Figure Lengend Snippet: Analysis of 23BD production in engineered strains of C. thermocellum . All strains were cultured in MTC-5 medium containing 5 g/L cellobiose as carbon source. (A) 23BD titers detected in the fermentation broth are the sum of both SS/RR 23BD and meso-23BD. (B) ALDC enzyme activity. (C) BDH enzyme activity. For both ALDC and BDH, assay conditions are described in the materials and methods section. Error bars represent the range of data, n = 2 biological replicates. Individual replicate data, and separate quantification of 23BD isomers, is available in Supporting Dataset D2. ∗AG8235 is the parent strain used to construct all the other strains listed in this table Δhpt Δ0478 Δ2366::polyattB. #1 – p1194, promoter from the Clo1313_1194 gene #2 – aggggga ribosome binding site sequence #3 – aggagga ribosome binding site sequence #4 – P_Clo1313_1194 (1st gene in the operon).

Article Snippet: They were checked for plasmid integration by PCR and whole genome sequencing by Plasmidsaurus Inc.

Techniques: Cell Culture, Activity Assay, Construct, Binding Assay, Sequencing

Journal: iScience

Article Title: Genomic and phenotypic comparisons reveal lineage-specific traits of the probiotic Saccharomyces boulardii

doi: 10.1016/j.isci.2026.115706

Figure Lengend Snippet: Comparative genomic analysis of Saccharomyces strains (A) Pangenome analysis of eight Saccharomyces strains using anvi’o. The central dendrogram reflects hierarchical clustering of gene clusters based on their presence or absence across genomes. Each concentric layer corresponds to a single genome, with colored bars marking the presence of a given gene cluster. Clusters are classified as single-copy core genes (SCGs; present in all genomes), accessory (present in 2–7 genomes), or singletons (strain-specific). Green rings denote clusters with functional annotations in either KOfam or Pfam databases. The outermost ring shows the combined homogeneity index, which summarizes sequence conservation across genomes within each cluster based on amino acid identity. (B) Whole-genome alignment of S. boulardii CNCM I-3799 to the S. cerevisiae reference strain S288C reveals a conserved 68.3 kb inversion on chromosome XVI (highlighted in orange). Gray blocks indicate syntenic regions. This inversion was present in all S. boulardii isolates and absent in all S. cerevisiae strains (see for other isolates).

Article Snippet: • The whole-genome sequencing for this study has been deposited in the National Center for Biotechnology Information (NCBI) under accession number PRJNA1312645: https://www.ncbi.nlm.nih.gov/sra/PRJNA1312645 .

Techniques: Functional Assay, Sequencing